- Description
| Specificity Profile | More Information |
|
The specificity of RSV-F Monoclonal Antibody (CSB0017) was tested on the Membrane Proteome Array® and shown to be specific for RSV-F. The Membrane Proteome Array® contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array® represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
|
(Left) COS-7 cells transiently transfected RSV-F were stained with RSV-F Monoclonal Antibody (CSB0017) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with RSV-F Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with RSV-F and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
|
(Left) COS-7 cells transiently transfected RSV-F were permeabilized and stained with RSV-F Monoclonal Antibody (CSB0017) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with RSV-F Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with RSV-F and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies
in-house so that you can confidently choose the right antibody for your assay. All antibodies are
VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and
SPECIFIC in binding to only your target.
| Target | RSV-F |
| Target Aliases | None |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Virus |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
RSV-F is a fusion glycoprotein that facilitates infection with respiratory syncytial virus (RSV). During infection, RSV-F mediates fusion between infected cells, forming syncytia. (UniProtKB/Swiss-Prot: P03420)
