- Description
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | PDCD1 |
| Target Aliases | PD1, PDCD1, CD279, HSLE1, SLEB2, HPD-1, HPD-L |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (other untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
| Specificity Profile | More Information |
 | The specificity of PDCD1 Monoclonal Antibody (CSB0023) was tested on the Membrane Proteome Array and shown to be specific for human PDCD1. The Membrane Proteome Array contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/mL |
 | HEK-293F cells transiently transfected with human PDCD1 were stained with PDCD1 Monoclonal Antibody (CSB0023) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with PDCD1 Monoclonal Antibody (CSB0023) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/mL |
 | (A) COS-7 cells transiently transfected with human PDCD1 were stained with PDCD1 Monoclonal Antibody (CSB0023) followed by AlexaFluor 647 anti-mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with PDCD1 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human PDCD1 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% triton | 1 µg/mL |
 | (A) COS-7 cells transiently transfected with human PDCD1 were permeabilized and stained with PDCD1 Monoclonal Antibody (CSB0023) followed by AlexaFluor 647 anti-mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with PDCD1 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human PDCD1 and stained with control MAb. | |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
PD-1 is single-pass transmembrane protein and an immune-inhibitory receptor on activated T-cells. PD-1 plays a role in regulation of T-cell functions and is important for protecting against autoimmunity. PD-1 can also inhibit anti-microbial and anti-tumor immunity, and is expressed in various tumors, including melanomas. (NCBI Gene: 5133, UniProtKB/Swiss-Prot: Q15116) Other names: PD1, PDCD1, CD279, HSLE1, SLEB2, HPD-1, HPD-L
