Flow Cytometry (Extracellular): HEK-293F cells transiently transfected with human KCNK4 were stained with KCNK4 Monoclonal Antibody (CSB0127) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with KCNK4 Monoclonal Antibody (CSB0127) (blue).

KCNK4 MAb - CSB0127

Catalog #: CSB0127
Applications: FC   IF   ICC   ELISA   WB  
Isotype: Mouse IgG2a
Reactivity: Human (others untested)
Concentration: 1 mg/ml
Size: 100 µg
Technical Data Sheet
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$475.00
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  • Description
Description
Specificity ProfileMore Information

The specificity of KCNK4 Monoclonal Antibody (CSB0127) was tested on the Membrane Proteome Array™ and shown to be specific for human KCNK4.

The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands.

ApplicationConditionsRecommended Concentration
Flow Cytometry, ExtracellularLive, Unpermeabilized1 µg/ml
HEK-293F cells transiently transfected with human KCNK4 were stained with KCNK4 Monoclonal Antibody (CSB0127) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with KCNK4 Monoclonal Antibody (CSB0127) (blue).
ApplicationConditionsRecommended Concentration
Immunofluorescence, ExtracellularFixed 4% paraformaldehyde1 µg/ml
(A) COS-7 cells transiently transfected with human KCNK4 were stained with KCNK4 Monoclonal Antibody (CSB0127) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with KCNK4 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human KCNK4 and stained with control MAb.
ApplicationConditionsRecommended Concentration
Immunofluorescence, IntracellularFixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-1001 µg/ml
(A) COS-7 cells transiently transfected with human KCNK4 were permeabilized and stained with KCNK4 Monoclonal Antibody (CSB0127) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with KCNK4 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human KCNK4 and stained with control MAb.

CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.

TargetKCNK4
Target AliasesTRAAK, K2p4.1, TRAAK1, FHEIG
IsotypeMouse IgG2a
Membrane Proteome SpecificityMonospecific for 6,000 membrane proteins tested
Species ReactivityHuman (others untested)
Fc ModificationsC-terminal Avitag, disabled Fc-gamma receptor binding
SourceRecombinant CHO expression; purified by Protein A chromatography
FormulationEndotoxin Free PBS pH 7.4, sterile-filtered
Concentration1 mg/ml

Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.

KCNK4 is a multi-pass transmembrane protein and TWIK-related arachidonic acid-stimulated two pore potassium channel. KCNK4 requires basic pH to open and opens more frequently at higher temperatures. KCNK4 is involved in pain perception. (NCBI Gene: 50801, UniProtKB/Swiss-Prot: Q9NYG8). Other names: TRAAK, K2p4.1, TRAAK1, FHEIG

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