- Description
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | IL2RA |
| Target Aliases | CD25, IDDM10, IL2R, P55, IMD41, TCGFR |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (other untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
| Specificity Profile | More Information |
 | The specificity of IL2RA Monoclonal Antibody (CSB0015) was tested on the Membrane Proteome Array and shown to be specific for human IL2RA. The Membrane Proteome Array contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/mL |
 | HEK-293F cells transiently transfected with human IL2RA were stained with IL2RA Monoclonal Antibody (CSB0015) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with IL2RA Monoclonal Antibody (CSB0015) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/mL |
 | (A) COS-7 cells transiently transfected with human IL2RA were stained with IL2RA Monoclonal Antibody (CSB0015) followed by AlexaFluor 647 anti-mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with IL2RA Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human IL2RA and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% triton | 1 µg/mL |
 | (A) COS-7 cells transiently transfected with human IL2RA were permeabilized and stained with IL2RA Monoclonal Antibody (CSB0015) followed by AlexaFluor 647 anti-mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with IL2RA Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human IL2RA and stained with control MAb. | |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
CD25 (also known as IL2-Ra) is a single-pass transmembrane protein and the alpha chain of the high-affinity IL2 receptor, which also includes beta and gamma chains. CD25 is involved in the regulation of immune tolerance. Severe COVID-19 causes elevated levels of IL2R in plasma, and elevated IL2R in serum is associated with some types of carcinomas. (NCBI Gene: 3559, UniProtKB/Swiss-Prot: P01589) This antibody uses the same variable regions as the clinical Basiliximab antibody. Basiliximab is indicated after kidney transplants for prevention of immediate transplant rejection (https://medlineplus.gov/druginfo/meds/a612013.html). Other names: CD25, IDDM10, IL2R, P55, IMD41, TCGFR
