- Description
| Specificity Profile | More Information |
 | The specificity of GLUT4 Monoclonal Antibody (CSB0152) was tested on the Membrane Proteome Array™ and shown to be specific for human GLUT4. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
 | HEK-293F cells transiently transfected with human GLUT4 were stained with GLUT4 Monoclonal Antibody (CSB0152) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-Human IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with GLUT4 Monoclonal Antibody (CSB0152) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
 | (A) COS-7 cells transiently transfected with human GLUT4 were stained with GLUT4 Monoclonal Antibody (CSB0152) followed by AlexaFluor 647 anti-Human IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with GLUT4 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human GLUT4 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
 | (A) COS-7 cells transiently transfected with human GLUT4 were permeabilized and stained with GLUT4 Monoclonal Antibody (CSB0152) followed by AlexaFluor 647 anti-Human IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with GLUT4 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human GLUT4 and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | GLUT4 |
| Target Aliases | SLC2A4, GLUT-4 |
| Isotype | Human IgG4 |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human, Mouse |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
GLUT4 is a multi-pass membrane protein, insulin-regulated facilitative glucose transporter, and member of solute carrier family 2. GLUT4 is stored in muscle and fat cells, and insulin stimulation causes GLUT4 to move to the cell membrane to transport glucose into the cell. Mutations in the GLUT4 gene are associated with type 2 diabetes. (NCBI Gene: 6517, UniProtKB/Swiss-Prot: P14672) Other names: SLC2A4, GLUT-4
Diaz-Vegas A, Norris DM, Jall-Rogg S, Cooke KC, Conway OJ, Shun-Shion AS, Duan X, Potter M, van Gerwen J, Baird HJ, Humphrey SJ, James DE, Fazakerley DJ, Burchfield JG. A high-content endogenous GLUT4 trafficking assay reveals new aspects of adipocyte biology. Life Sci Alliance. 2022 Oct 25;6(1):e202201585. doi: 10.26508/lsa.202201585. PMID: 36283703; PMCID: PMC9595207.
Duan X, Norris DM, Humphrey SJ, Yang P, Cooke KC, Bultitude WP, Parker BL, Conway OJ, Burchfield JG, Krycer JR, Brodsky FM, James DE, Fazakerley DJ. Trafficking regulator of GLUT4-1 (TRARG1) is a GSK3 substrate. Biochem J. 2022 Jun 17;479(11):1237-1256. doi: 10.1042/BCJ20220153. PMID: 35594055; PMCID: PMC9284383.
Tucker DF, Sullivan JT, Mattia KA, Fisher CR, Barnes T, Mabila MN, Wilf R, Sulli C, Pitts M, Payne RJ, Hall M, Huston-Paterson D, Deng X, Davidson E, Willis SH, Doranz BJ, Chambers R, Rucker JB. Isolation of state-dependent monoclonal antibodies against the 12-transmembrane domain glucose transporter 4 using virus-like particles. Proc Natl Acad Sci U S A. 2018 May 29;115(22):E4990-E4999. doi: 10.1073/pnas.1716788115. Epub 2018 May 16. PMID: 29769329; PMCID: PMC5984492.
