- Description
| Specificity Profile | More Information |
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The specificity of EGFR Monoclonal Antibody (CSB0016) was tested on the Membrane Proteome Array® and shown to be specific for human EGFR. The Membrane Proteome Array® contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array® represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
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JS-1 cells transiently transfected with human EGFR were stained with EGFR Monoclonal Antibody (CSB0016) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti-Mouse IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with EGFR Monoclonal Antibody (CSB0016) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
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(Left) JS-1 cells transiently transfected human EGFR were stained with EGFR Monoclonal Antibody (CSB0016) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with EGFR Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human EGFR and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
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(Left) JS-1 cells transiently transfected human EGFR were permeabilized and stained with EGFR Monoclonal Antibody (CSB0016) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with EGFR Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human EGFR and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies
in-house so that you can confidently choose the right antibody for your assay. All antibodies are
VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and
SPECIFIC in binding to only your target.
| Target | EGFR |
| Target Aliases | ERBB1, ERRP, ERBB, HER1, EGFR VIII, NISBD2, PIG61, MENA |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (Others untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
EGFR is a single-pass transmembrane protein and member of the protein kinase superfamily that binds proteins in the epidermal growth factor family, causing receptor dimerization and tyrosine autophosphorylation, which activate signaling cascades. EGFR is involved in cell proliferation and migration and plays a role in hepatitis C infection and cytokine storm in severe COVID-19. Mutations in the EGFR gene are also associated with lung cancer. (NCBI Gene: 1956, UniProtKB/Swiss-Prot: P00533) Other names: ERBB1, ERRP, ERBB, HER1, EGFR VIII, NISBD2, PIG61, MENA
