- Description
| Specificity Profile | More Information |
 | The specificity of Chikungunya Virus E1/E2 Monoclonal Antibody (CSB0125) was tested on the Membrane Proteome Array™ and shown to be specific for human Chikungunya Virus E1/E2. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
 | HEK-293F cells transiently transfected with Chikungunya Virus E1/E2 were stained with the Chikungunya Virus E1/E2 Monoclonal Antibody (CSB0125) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with the Chikungunya Virus E1/E2 Monoclonal Antibody (CSB0125) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
 | (A) COS-7 cells transiently transfected with Chikungunya Virus E1/E2 were stained with the Chikungunya Virus E1/E2 Monoclonal Antibody (CSB0125) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with the Chikungunya Virus E1/E2 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with Chikungunya Virus E1/E2 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
 | (A) COS-7 cells transiently transfected with Chikungunya Virus E1/E2 were permeabilized and stained with the Chikungunya Virus E1/E2 Monoclonal Antibody (CSB0125) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with the Chikungunya Virus E1/E2 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with Chikungunya Virus E1/E2 and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | Chikungunya Virus E1/E2 |
| Target Aliases | |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Chikungunya Virus |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
Chikungunya virus (CHIKV) is spread through bites from infected mosquitoes and can cause fever, joint pain and swelling, headache, muscle pain, or a rash (https://www.cdc.gov/chikungunya/index.html).
Fong RH, Banik SS, Mattia K, Barnes T, Tucker D, Liss N, Lu K, Selvarajah S, Srinivasan S, Mabila M, Miller A, Muench MO, Michault A, Rucker JB, Paes C, Simmons G, Kahle KM, Doranz BJ. Exposure of epitope residues on the outer face of the chikungunya virus envelope trimer determines antibody neutralizing efficacy. J Virol. 2014 Dec;88(24):14364-79. doi: 10.1128/JVI.01943-14. Epub 2014 Oct 1. PMID: 25275138.
