Flow Cytometry (Extracellular): HEK-293F cells transiently transfected with human CD28 were stained with CD28 Monoclonal Antibody (CSB0012) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with CD28 Monoclonal Antibody (CSB0012) (blue).

CD28 MAb - CSB0012

Catalog #: CSB0012
Applications: FC   IF   ICC   ELISA   WB  
Isotype: Mouse IgG2a
Reactivity: Human (others untested)
Concentration: 1 mg/ml
Size: 100 µg
Technical Data Sheet
Safety Data Sheet
$475.00
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  • Description
Description
Specificity ProfileMore Information

The specificity of CD28 Monoclonal Antibody (CSB0012) was tested on the Membrane Proteome Array™ and shown to be specific for human CD28.

The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands.

ApplicationConditionsRecommended Concentration
Flow Cytometry, ExtracellularLive, Unpermeabilized1 µg/ml
HEK-293F cells transiently transfected with human CD28 were stained with CD28 Monoclonal Antibody (CSB0012) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with CD28 Monoclonal Antibody (CSB0012) (blue).
ApplicationConditionsRecommended Concentration
Immunofluorescence, ExtracellularFixed 4% paraformaldehyde1 µg/ml
(A) COS-7 cells transiently transfected with human CD28 were stained with CD28 Monoclonal Antibody (CSB0012) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with CD28 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human CD28 and stained with control MAb.
ApplicationConditionsRecommended Concentration
Immunofluorescence, IntracellularFixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-1001 µg/ml
(A) COS-7 cells transiently transfected with human CD28 were permeabilized and stained with CD28 Monoclonal Antibody (CSB0012) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with CD28 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human CD28 and stained with control MAb.

CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.

TargetCD28
Target AliasesTP44
IsotypeMouse IgG2a
Membrane Proteome SpecificityMonospecific for 6,000 membrane proteins tested
Species ReactivityHuman (others untested)
Fc ModificationsC-terminal Avitag, disabled Fc-gamma receptor binding
SourceRecombinant CHO expression; purified by Protein A chromatography
FormulationEndotoxin Free PBS pH 7.4, sterile-filtered
Concentration1 mg/ml

Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.

CD28 is a single-pass transmembrane protein and cell surface glycoprotein that is required for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. (NCBI Gene: 940, UniProtKB/Swiss-Prot: P10747). Other names: TP44

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