- Description
| Specificity Profile | More Information |
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The specificity of CD1D Monoclonal Antibody (CSB0094) was tested on the Membrane Proteome Array® and shown to be specific for human CD1D. The Membrane Proteome Array® contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array® represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
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(Left) COS-7 cells transiently transfected human CD1D were stained with CD1D Monoclonal Antibody (CSB0094) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with CD1D Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human CD1D and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
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(Left) COS-7 cells transiently transfected human CD1D were permeabilized and stained with CD1D Monoclonal Antibody (CSB0094) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with CD1D Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human CD1D and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies
in-house so that you can confidently choose the right antibody for your assay. All antibodies are
VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and
SPECIFIC in binding to only your target.
| Target | CD1D |
| Target Aliases | R3G1, CD1A, R3 |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (Others untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
CD1D is a single-pass transmembrane protein that is part of the CD1 family of transmembrane glycoproteins. CD1D, like other members of the CD1 family, binds and presents self and microbial glycolipids to T-cells. (NCBI Gene: 912, UniProtKB/Swiss-Prot: P15813) Other names: R3G1, CD1A, R3
