- Description
| Specificity Profile | More Information |
|
The specificity of CD151 Monoclonal Antibody (CSB0001) was tested on the Membrane Proteome Array® and shown to be specific for human CD151. The Membrane Proteome Array® contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array® represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
|
JS-1 cells transiently transfected with human CD151 were stained with CD151 Monoclonal Antibody (CSB0001) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti-Mouse IgG secondary antibody. JS-1 cells transiently transfected with an empty control vector were also stained with CD151 Monoclonal Antibody (CSB0001) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
|
(Left) JS-1 cells transiently transfected human CD151 were stained with CD151 Monoclonal Antibody (CSB0001) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CD151 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human CD151 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
|
(Left) JS-1 cells transiently transfected human CD151 were permeabilized and stained with CD151 Monoclonal Antibody (CSB0001) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) JS-1 cells transiently transfected with an empty control vector stained with CD151 Monoclonal Antibody. (Bottom right) Isotype control: JS-1 cells transfected with human CD151 and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies
in-house so that you can confidently choose the right antibody for your assay. All antibodies are
VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and
SPECIFIC in binding to only your target.
| Target | CD151 |
| Target Aliases | TSPAN24, PETA-3, RAPH, SFA-1, GP27, EBS7, MER2 |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (Others untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
CD151 is a member of the tetraspanin family, which is typically characterized by four hydrophobic domains. CD151 complexes with integrins and other tetraspanins, is involved in cell adhesion, and may also regulate integrin trafficking and function. CD151 plays a role in HPV-16 endocytosis and cell motility, invasion, and metastasis of cancer cells. (NCBI Gene: 977, UniProtKB/Swiss-Prot: P48509) Other names: TSPAN24, PETA-3, RAPH, SFA-1, GP27, EBS7, MER2
