- Description
| Specificity Profile | More Information |
 | The specificity of C5AR1 Monoclonal Antibody (CSB0027) was tested on the Membrane Proteome Array™ and shown to be specific for human C5AR1. The Membrane Proteome Array™ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array™ represents the industry standard for determining the binding specificity of antibodies and other protein ligands. |
| Application | Conditions | Recommended Concentration |
| Flow Cytometry, Extracellular | Live, Unpermeabilized | 1 µg/ml |
 | HEK-293F cells transiently transfected with human C5AR1 were stained with C5AR1 Monoclonal Antibody (CSB0027) (green) or isotype control antibody (gray), followed by AlexaFluor 647-conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with C5AR1 Monoclonal Antibody (CSB0027) (blue). | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Extracellular | Fixed 4% paraformaldehyde | 1 µg/ml |
 | (A) COS-7 cells transiently transfected with human C5AR1 were stained with C5AR1 Monoclonal Antibody (CSB0027) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with C5AR1 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human C5AR1 and stained with control MAb. | |
| Application | Conditions | Recommended Concentration |
| Immunofluorescence, Intracellular | Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100 | 1 µg/ml |
 | (A) COS-7 cells transiently transfected with human C5AR1 were permeabilized and stained with C5AR1 Monoclonal Antibody (CSB0027) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (B) COS-7 cells transiently transfected with an empty control vector stained with C5AR1 Monoclonal Antibody. (C) Isotype control: COS-7 cells transfected with human C5AR1 and stained with control MAb. | |
CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.
| Target | C5AR1 |
| Target Aliases | C5AR, CD88, C5R1, C5A, C5a-R, C5aR |
| Isotype | Mouse IgG2a |
| Membrane Proteome Specificity | Monospecific for 6,000 membrane proteins tested |
| Species Reactivity | Human (others untested) |
| Fc Modifications | C-terminal Avitag, disabled Fc-gamma receptor binding |
| Source | Recombinant CHO expression; purified by Protein A chromatography |
| Formulation | Endotoxin Free PBS pH 7.4, sterile-filtered |
| Concentration | 1 mg/ml |
Stable for 12 months from date of receipt when stored at 4°C. Avoid repeated freeze-thaw cycles.
C5AR1 is a multi-pass transmembrane protein and a receptor for C5a, which is a chemotactic and inflammatory peptide anaphylatoxin. C5AR1 is involved in the complement component C5a signaling pathway and RNA polymerase II transcription and positively regulates the ERK1/2 cascade. C5AR1 is a biomarker for asthma, chronic obstructive pulmonary disease, severe acute respiratory syndrome, rhinitis, and Alzheimer’s disease. (NCBI Gene: 728, UniProtKB/Swiss-Prot: P21730) Other names: C5AR, CD88, C5R1, C5A, C5a-R, C5aR
