APOE MAb - CSB0031 - Cell Surface Bio

Description
Validated Applications
Stability & Storage
Background
Publications

Description

CSB VeRSaMAbs:
We produce and validate all our monoclonal antibodies in-house so that you can confidently choose the right antibody for your assay. All antibodies are VALIDATED for common applications, RECOMBINANT for batch-to-batch consistency, and SPECIFIC in binding to only your target.

Target	APOE
Target Aliases	AD2, LDLCQ5, APO-E, ApoE4, Apo-E, LPG, Apolipoprotein E, Apolipoprotein E3
Isotype	Mouse IgG2a
Membrane Proteome Specificity	Monospecific for 6,000 membrane proteins tested
Species Reactivity	Human (Others untested)
Fc Modifications	C-terminal Avitag, disabled Fc-gamma receptor binding
Source	Recombinant CHO expression; purified by Protein A chromatography
Formulation	Endotoxin Free PBS pH 7.4, sterile-filtered
Concentration	1 mg/ml

Validated Applications

Specificity Profile

More Information

The specificity of APOE Monoclonal Antibody (CSB0031) was tested on the Membrane Proteome Array® and shown to be specific for human APOE.

The Membrane Proteome Array® contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Array® represents the industry standard for determining the binding specificity of antibodies and other protein ligands.

Application	Conditions	Recommended Concentration
Flow Cytometry, Extracellular	Live, Unpermeabilized	1 µg/ml
	HEK-293F cells transiently transfected with human APOE were stained with APOE Monoclonal Antibody (CSB0031) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with APOE Monoclonal Antibody (CSB0031) (blue).

Application	Conditions	Recommended Concentration
Immunofluorescence, Extracellular	Fixed 4% paraformaldehyde	1 µg/ml
	(Left) COS-7 cells transiently transfected human APOE were stained with APOE Monoclonal Antibody (CSB0031) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with APOE Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human APOE and stained with control MAb.

Application	Conditions	Recommended Concentration
Immunofluorescence, Intracellular	Fixed 4% paraformaldehyde, Permeabilized 0.1% Triton X-100	1 µg/ml
	(Left) COS-7 cells transiently transfected human APOE were permeabilized and stained with APOE Monoclonal Antibody (CSB0031) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with APOE Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human APOE and stained with control MAb.

Stability & Storage Background

Catalog #:	CSB0031
Applications:	FC IF ICC ELISA WB
Isotype:	Mouse IgG2a
Reactivity:	Human (Others untested)
Extracellular epitope:	Yes
Concentration:	1 mg/ml
Size:	100 µg
Fixative Compatibility:	Paraformaldehyde (4%, IF)
Detergent Compatibility:	Triton X-100 (0.1%, IF)